› 5me6

X-ray diffraction
2.9Å resolution

Crystal Structure of eiF4E from C. melo bound to a CAP analog

Released:
DOI: 10.2210/pdb5me6/pdb
PDB entry 5me6 coloured by chain, front view.
PDB entry 5me6 coloured by chain, side view.
PDB entry 5me6 coloured by chain, top view.
Source organism: Cucumis melo
Primary publication:
Structure of eIF4E in Complex with an eIF4G Peptide Supports a Universal Bipartite Binding Mode for Protein Translation.
Miras M, Truniger V, Silva C, Verdaguer N, Aranda MA, Querol-Audí J
Plant Physiol 174 1476-1491 (2017)
PMID: 28522457

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169506 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Eukaryotic translation initiation factor 4E-1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 186 amino acids
Theoretical weight: 21.35 KDa
Source organism: Cucumis melo
Expression system: Escherichia coli
UniProt:
  • Canonical: Q00LS8 (Residues: 53-235; Coverage: 78%)
Gene names: E6C27_scaffold96G002720, eIF4E
Sequence domains: Eukaryotic initiation factor 4E
Structure domains: RNA Cap, Translation Initiation Factor Eif4e

Ligands and Environments

1 bound ligand:
Ligand M7G 2 x M7G
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P212121
Unit cell:
a: 57.699Å b: 108.868Å c: 122.839Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.22 0.263
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The Organizing Principles of Eukaryotic Ribosome Recruitment.
Pelletier et al. (2019)
4 more