› 5mec

X-ray diffraction
2.13Å resolution

Crystal structure of yeast Cdt1 middle domain (residues 294-433)

Released:
DOI: 10.2210/pdb5mec/pdb
PDB entry 5mec coloured by chain, front view.
PDB entry 5mec coloured by chain, side view.
PDB entry 5mec coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Cdt1 stabilizes an open MCM ring for helicase loading.
Frigola J, He J, Kinkelin K, Pye VE, Renault L, Douglas ME, Remus D, Cherepanov P, Costa A, Diffley JFX
Nat Commun 8 15720 (2017)
PMID: 28643783

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155600 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cell division cycle protein CDT1 Chain: A
Molecule details ›
Chain: A
Length: 169 amino acids
Theoretical weight: 19.28 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P47112 (Residues: 272-438; Coverage: 28%)
Gene names: CDT1, J1641, SID2, TAH11, YJR046W

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 45.13Å b: 46.33Å c: 75.36Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.196 0.244
Expression system: Escherichia coli

Quick links

Citations

10 review citations

The plasticity of DNA replication forks in response to clinically relevant genotoxic stress.
Berti et al. (2020)
9 more

31 mentions without citation

Epigenetics and bacterial infections.
Bierne et al. (2012)
30 more