› 5mf1

X-ray diffraction
3.3Å resolution

Crystal structure of a C-terminally truncated trimeric ectodomain of the Chlamydomonas reinhardtii gamete fusion protein HAP2

Released:
DOI: 10.2210/pdb5mf1/pdb
PDB entry 5mf1 coloured by chain, front view.
PDB entry 5mf1 coloured by chain, side view.
PDB entry 5mf1 coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
The Ancient Gamete Fusogen HAP2 Is a Eukaryotic Class II Fusion Protein.
Fédry J, Liu Y, Péhau-Arnaudet G, Pei J, Li W, Tortorici MA, Traincard F, Meola A, Bricogne G, Grishin NV, Snell WJ, Rey FA, Krey T
Cell 168 904-915.e10 (2017)
PMID: 28235200

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107295 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hapless 2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 611 amino acids
Theoretical weight: 64.42 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: A4GRC6 (Residues: 23-592; Coverage: 51%)
Gene names: GCS1, HAP2
Sequence domains: Male gamete fusion factor

Ligands and Environments

2 bound ligands:
Ligand NAG 3 x NAG
Ligand NGA 3 x NGA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: P212121
Unit cell:
a: 103.97Å b: 114.06Å c: 137.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.219 0.266
Expression system: Drosophila melanogaster

Quick links

Citations

32 review citations

Origin of viruses: primordial replicators recruiting capsids from hosts.
Krupovic et al. (2019)
31 more

7 mentions without citation

The Ancient Gamete Fusogen HAP2 Is a Eukaryotic Class II Fusion Protein.
Fédry et al. (2017)
6 more