› 5mfh

X-ray diffraction
2Å resolution

Designed armadillo repeat protein YIIIM5AII in complex with peptide (RR)5

Released:
DOI: 10.2210/pdb5mfh/pdb
Model geometry
Fit model/data
PDB entry 5mfh coloured by chain, front view.
PDB entry 5mfh coloured by chain, side view.
PDB entry 5mfh coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Curvature of designed armadillo repeat proteins allows modular peptide binding.
Hansen S, Ernst P, König SLB, Reichen C, Ewald C, Nettels D, Mittl PRE, Schuler B, Plückthun A
J Struct Biol 201 108-117 (2018)
PMID: 28864298

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-165991 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
YIIIM5AII Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 286 amino acids
Theoretical weight: 30.03 KDa
Source organism: synthetic construct
Expression system: Escherichia coli K-12
Structure domains: Leucine-rich Repeat Variant
(RR)5 Chains: E, F
Molecule details ›
Chains: E, F
Length: 10 amino acids
Theoretical weight: 1.59 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 21 x CA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 81.48Å b: 103.79Å c: 134.01Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.199 0.232
Expression systems:
  • Escherichia coli K-12
  • Not provided

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Citations

1 review citation

Toxin Neutralization Using Alternative Binding Proteins.
Jenkins et al. (2019)
5 other articles