› 5mfn

X-ray diffraction
2.8Å resolution

Designed armadillo repeat protein YIIIM5AII

Released:
DOI: 10.2210/pdb5mfn/pdb
PDB entry 5mfn coloured by chain, front view.
PDB entry 5mfn coloured by chain, side view.
PDB entry 5mfn coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Curvature of designed armadillo repeat proteins allows modular peptide binding.
Hansen S, Ernst P, König SLB, Reichen C, Ewald C, Nettels D, Mittl PRE, Schuler B, Plückthun A
J Struct Biol 201 108-117 (2018)
PMID: 28864298

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165997 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
YIIIM5AII Chains: A, B
Molecule details ›
Chains: A, B
Length: 286 amino acids
Theoretical weight: 30.03 KDa
Source organism: synthetic construct
Expression system: Escherichia coli K-12

Ligands and Environments

2 bound ligands:
Ligand CA 3 x CA
Ligand MLT 2 x MLT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P6322
Unit cell:
a: 142.55Å b: 142.55Å c: 142.12Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.2 0.246
Expression system: Escherichia coli K-12

Quick links

Citations

1 review citation

Toxin Neutralization Using Alternative Binding Proteins.
Jenkins et al. (2019)
3 other articles

2 mentions without citation

An automated iterative approach for protein structure refinement using pseudocontact shifts.
Cucuzza et al. (2021)
1 more