› 5mhj

X-ray diffraction
2.12Å resolution

ICP4 DNA-binding domain, lacking intrinsically disordered region, in complex with 12mer DNA duplex from its own promoter

Released:
DOI: 10.2210/pdb5mhj/pdb
PDB entry 5mhj coloured by chain, front view.
PDB entry 5mhj coloured by chain, side view.
PDB entry 5mhj coloured by chain, top view.
Source organisms:
Primary publication:
The herpes viral transcription factor ICP4 forms a novel DNA recognition complex.
Tunnicliffe RB, Lockhart-Cairns MP, Levy C, Mould AP, Jowitt TA, Sito H, Baldock C, Sandri-Goldin RM, Golovanov AP
Nucleic Acids Res 45 8064-8078 (2017)
PMID: 28505309

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119024 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Major viral transcription factor ICP4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 201 amino acids
Theoretical weight: 21.31 KDa
Source organism: Human alphaherpesvirus 1 strain 17
Expression system: Escherichia coli
UniProt:
  • Canonical: P08392 (Residues: 288-487; Coverage: 15%)
Gene names: ICP4, IE175, RS1
Sequence domains: Herpesvirus ICP4-like protein N-terminal region
DNA (5'-D(P*CP*GP*AP*TP*CP*GP*TP*CP*C)-3') Chain: E
Molecule details ›
Chain: E
Length: 12 nucleotides
Theoretical weight: 3.58 KDa
Source organism: Human alphaherpesvirus 1
Expression system: Not provided
DNA (5'-D(P*CP*GP*AP*TP*CP*GP*TP*CP*C)-3') Chain: F
Molecule details ›
Chain: F
Length: 12 nucleotides
Theoretical weight: 3.74 KDa
Source organism: Human alphaherpesvirus 1
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ACY 2 x ACY
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 127.254Å b: 39.084Å c: 90.443Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.235
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Deep structural insights into RNA-binding disordered protein regions.
Zeke et al. (2022)
2 more

2 mentions without citation

The herpes viral transcription factor ICP4 forms a novel DNA recognition complex.
Tunnicliffe et al. (2017)
1 more