› 5mif

X-ray diffraction
2.14Å resolution

Crystal structure of carboxyl esterase 2 (TmelEST2) from mycorrhizal fungus Tuber melanosporum

Released:
DOI: 10.2210/pdb5mif/pdb
PDB entry 5mif coloured by chain, front view.
PDB entry 5mif coloured by chain, side view.
PDB entry 5mif coloured by chain, top view.
Source organism: Tuber melanosporum Mel28
Primary publication:
A family of archaea-like carboxylesterases preferentially expressed in the symbiotic phase of the mychorrizal fungus Tuber melanosporum.
Cavazzini D, Grossi G, Levati E, Vallese F, Montanini B, Bolchi A, Zanotti G, Ottonello S
Sci Rep 7 7628 (2017)
PMID: 28794466

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112692 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha/beta hydrolase fold-3 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 347 amino acids
Theoretical weight: 37.44 KDa
Source organism: Tuber melanosporum Mel28
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D5GA36 (Residues: 1-347; Coverage: 100%)
Gene name: GSTUM_00003552001
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand TRT 2 x TRT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P6522
Unit cell:
a: 158.898Å b: 158.898Å c: 226.809Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.18 0.208
Expression system: Escherichia coli BL21(DE3)

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Citations

5 citation in other articles

C-Terminal β8-α9 Interaction Modulates Thermal Stability and Enzymatic Activity Differently in Hyperthermophilic Esterase EstE1 and Mesophilic Esterase rPPE.
Nguyen et al. (2023)
4 more

2 mentions without citation

A family of archaea-like carboxylesterases preferentially expressed in the symbiotic phase of the mychorrizal fungus Tuber melanosporum.
Cavazzini et al. (2017)
1 more