› 5mjk

X-ray diffraction
2Å resolution

Crystal Structure of Lactococcus lactis Thioredoxin Reductase (FO conformation)

Released:
DOI: 10.2210/pdb5mjk/pdb
PDB entry 5mjk coloured by chain, front view.
PDB entry 5mjk coloured by chain, side view.
PDB entry 5mjk coloured by chain, top view.
Source organism: Lactococcus cremoris
Primary publication:
The structure of Lactococcus lactis thioredoxin reductase reveals molecular features of photo-oxidative damage.
Skjoldager N, Blanner Bang M, Rykær M, Björnberg O, Davies MJ, Svensson B, Harris P, Hägglund P
Sci Rep 7 46282 (2017)
PMID: 28397795

Function and Biology Details

Reaction catalysed: 
Thioredoxin + NADP(+) = thioredoxin disulfide + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106949 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin reductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 327 amino acids
Theoretical weight: 36.03 KDa
Source organism: Lactococcus cremoris
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A2RLJ5 (Residues: 1-308; Coverage: 100%)
Gene names: llmg_1588, trxB1
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments


Cofactor: Ligand FAD 4 x FAD
1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: P21
Unit cell:
a: 73.61Å b: 132.26Å c: 73.5Å
α: 90° β: 112.62° γ: 90°
R-values:
R R work R free
0.232 0.23 0.276
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Photo-Oxidation of Therapeutic Protein Formulations: From Radical Formation to Analytical Techniques.
Hipper et al. (2021)
2 other articles

1 mention without citation

The structure of Lactococcus lactis thioredoxin reductase reveals molecular features of photo-oxidative damage.
Skjoldager et al. (2017)