› 5mkg

X-ray diffraction
2.44Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166002 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Diguanylate phosphodiesterase Chain: A
Molecule details ›
Chain: A
Length: 256 amino acids
Theoretical weight: 28.41 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
Structure domains: EAL domain
Diguanylate phosphodiesterase Chain: B
Molecule details ›
Chain: B
Length: 254 amino acids
Theoretical weight: 28.21 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
Structure domains: EAL domain

Ligands and Environments

2 bound ligands:
Ligand CA 7 x CA
Ligand C2E 2 x C2E
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: C2
Unit cell:
a: 112.23Å b: 59.4Å c: 92.78Å
α: 90° β: 115.03° γ: 90°
R-values:
R R work R free
0.24 0.238 0.285
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 citation in other articles

Insights into Biofilm Dispersal Regulation from the Crystal Structure of the PAS-GGDEF-EAL Region of RbdA from Pseudomonas aeruginosa.
Liu et al. (2018)
9 more

1 mention without citation

Dimerisation induced formation of the active site and the identification of three metal sites in EAL-phosphodiesterases.
Bellini et al. (2017)