› 5mkk

X-ray diffraction
2.7Å resolution

Crystal structure of the heterodimeric ABC transporter TmrAB, a homolog of the antigen translocation complex TAP

Released:
DOI: 10.2210/pdb5mkk/pdb
PDB entry 5mkk coloured by chain, front view.
PDB entry 5mkk coloured by chain, side view.
PDB entry 5mkk coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
Crystal structure and mechanistic basis of a functional homolog of the antigen transporter TAP.
Nöll A, Thomas C, Herbring V, Zollmann T, Barth K, Mehdipour AR, Tomasiak TM, Brüchert S, Joseph B, Abele R, Oliéric V, Wang M, Diederichs K, Hummer G, Stroud RM, Pos KM, Tampé R
Proc Natl Acad Sci U S A 114 E438-E447 (2017)
PMID: 28069938

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180848 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Multidrug resistance ABC transporter ATP-binding and permease protein Chain: A
Molecule details ›
Chain: A
Length: 611 amino acids
Theoretical weight: 69.1 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q72J05 (Residues: 2-600; Coverage: 100%)
Gene name: TT_C0976
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Multidrug resistance ABC transporter ATP-binding and permease protein Chain: B
Molecule details ›
Chain: B
Length: 577 amino acids
Theoretical weight: 64.5 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q72J04 (Residues: 2-578; Coverage: 100%)
Gene name: TT_C0977
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P6522
Unit cell:
a: 93.371Å b: 93.371Å c: 1,043.95Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.226 0.272
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Emerging Diversity in Lipid-Protein Interactions.
Corradi et al. (2019)
8 more

11 mentions without citation

Structural and functional diversity calls for a new classification of ABC transporters.
Thomas et al. (2020)
10 more