› 5mn5

X-ray diffraction
2.8Å resolution

S. aureus FtsZ 12-316 T66W GTP Closed form (2TCm)

Released:
DOI: 10.2210/pdb5mn5/pdb
Model geometry
Fit model/data
PDB entry 5mn5 coloured by chain, front view.
PDB entry 5mn5 coloured by chain, side view.
PDB entry 5mn5 coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
A Polymerization-Associated Structural Switch in FtsZ That Enables Treadmilling of Model Filaments.
Wagstaff JM, Tsim M, Oliva MA, García-Sanchez A, Kureisaite-Ciziene D, Andreu JM, Löwe J
OpenAccess logo mBio 8 (2017)
PMID: 28465423

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140890 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cell division protein FtsZ Chains: A, B
Molecule details ›
Chains: A, B
Length: 305 amino acids
Theoretical weight: 31.61 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A031 (Residues: 12-316; Coverage: 78%)
Gene name: ftsZ
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GTP 2 x GTP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 43.781Å b: 59.85Å c: 187.664Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.217 0.263
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

At the Heart of Bacterial Cytokinesis: The Z Ring.
Du et al. (2019)
14 more

2 mentions without citation

A Polymerization-Associated Structural Switch in FtsZ That Enables Treadmilling of Model Filaments.
Wagstaff et al. (2017)
1 more