› 5mn7

X-ray diffraction
3.3Å resolution

S. aureus FtsZ 12-316 F138A GTP Closed form (3FCm)

Released:
DOI: 10.2210/pdb5mn7/pdb
PDB entry 5mn7 coloured by chain, front view.
PDB entry 5mn7 coloured by chain, side view.
PDB entry 5mn7 coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
A Polymerization-Associated Structural Switch in FtsZ That Enables Treadmilling of Model Filaments.
Wagstaff JM, Tsim M, Oliva MA, García-Sanchez A, Kureisaite-Ciziene D, Andreu JM, Löwe J
mBio 8 (2017)
PMID: 28465423

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140891 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cell division protein FtsZ Chains: A, B
Molecule details ›
Chains: A, B
Length: 305 amino acids
Theoretical weight: 31.45 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A031 (Residues: 12-316; Coverage: 78%)
Gene name: ftsZ
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand GTP 2 x GTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: I222
Unit cell:
a: 116.091Å b: 130.015Å c: 134.125Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.211 0.268
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

At the Heart of Bacterial Cytokinesis: The Z Ring.
Du et al. (2019)
14 more

2 mentions without citation

A Polymerization-Associated Structural Switch in FtsZ That Enables Treadmilling of Model Filaments.
Wagstaff et al. (2017)
1 more