› 5mnv

X-ray diffraction
2.97Å resolution

Structural and functional characterization of OleP in complex with 6DEB in PEG

Released:
DOI: 10.2210/pdb5mnv/pdb
PDB entry 5mnv coloured by chain, front view.
PDB entry 5mnv coloured by chain, side view.
PDB entry 5mnv coloured by chain, top view.
Source organism: Streptomyces antibioticus
Primary publication:
Substrate-induced conformational change in cytochrome P450 OleP.
Parisi G, Montemiglio LC, Giuffrè A, Macone A, Scaglione A, Cerutti G, Exertier C, Savino C, Vallone B
FASEB J 33 1787-1800 (2019)
PMID: 30207799

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176826 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P-450 Chains: A, B, C, D, E, F, G, H, I
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I
Length: 407 amino acids
Theoretical weight: 45.01 KDa
Source organism: Streptomyces antibioticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q59819 (Residues: 1-407; Coverage: 100%)
Gene name: oleP
Sequence domains: Cytochrome P450

Ligands and Environments

2 bound ligands:
Ligand HEM 9 x HEM
Ligand DEB 9 x DEB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P1
Unit cell:
a: 112.5Å b: 116.7Å c: 123.3Å
α: 103.9° β: 104.3° γ: 114.7°
R-values:
R R work R free
0.242 0.238 0.311
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Recent trends in biocatalysis.
Yi et al. (2021)
1 more

5 mentions without citation

Structural insights into an atypical secretory pathway kinase crucial for Toxoplasma gondii invasion.
Lentini et al. (2021)
4 more