› 5mpa

Electron Microscopy
4.5Å resolution

26S proteasome in presence of ATP (s2)

Released:
DOI: 10.2210/pdb5mpa/pdb
PDB entry 5mpa coloured by chain, front view.
PDB entry 5mpa coloured by chain, side view.
PDB entry 5mpa coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural insights into the functional cycle of the ATPase module of the 26S proteasome.
Wehmer M, Rudack T, Beck F, Aufderheide A, Pfeifer G, Plitzko JM, Förster F, Schulten K, Baumeister W, Sakata E
Proc Natl Acad Sci U S A 114 1305-1310 (2017)
PMID: 28115689
Related structures: EMD-3535

Function and Biology Details

Reaction catalysed: 
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 34-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149133 (preferred)
Entry contents:
20 distinct polypeptide molecules
Macromolecules (20 distinct):
Proteasome subunit alpha type-1 Chains: A, a
Molecule details ›
Chains: A, a
Length: 252 amino acids
Theoretical weight: 28.03 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P21243 (Residues: 1-252; Coverage: 100%)
Gene names: PRC2, PRS2, SCL1, YGL011C
Sequence domains:
Proteasome subunit alpha type-2 Chains: B, b
Molecule details ›
Chains: B, b
Length: 250 amino acids
Theoretical weight: 27.19 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P23639 (Residues: 1-250; Coverage: 100%)
Gene names: PRE8, PRS4, YML092C
Sequence domains:
Proteasome subunit alpha type-3 Chains: C, c
Molecule details ›
Chains: C, c
Length: 258 amino acids
Theoretical weight: 28.75 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P23638 (Residues: 1-258; Coverage: 100%)
Gene names: PRE9, PRS5, YGR135W
Sequence domains:
Proteasome subunit alpha type-4 Chains: D, d
Molecule details ›
Chains: D, d
Length: 254 amino acids
Theoretical weight: 28.48 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P40303 (Residues: 1-254; Coverage: 100%)
Gene names: PRE6, YOL038W
Sequence domains:
Proteasome subunit alpha type-5 Chains: E, e
Molecule details ›
Chains: E, e
Length: 260 amino acids
Theoretical weight: 28.65 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P32379 (Residues: 1-260; Coverage: 100%)
Gene names: DOA5, G9155, PUP2, YGR253C
Sequence domains:
Proteasome subunit alpha type-6 Chains: F, f
Molecule details ›
Chains: F, f
Length: 234 amino acids
Theoretical weight: 25.63 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P40302 (Residues: 1-234; Coverage: 100%)
Gene names: PRE5, YM9924.06, YMR314W
Sequence domains:
Probable proteasome subunit alpha type-7 Chains: G, g
Molecule details ›
Chains: G, g
Length: 288 amino acids
Theoretical weight: 31.58 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P21242 (Residues: 1-288; Coverage: 100%)
Gene names: O6650, PRC1, PRE10, PRS1, YOR362C
Sequence domains:
Proteasome subunit beta type-1 Chains: 1, h
Molecule details ›
Chains: 1, h
Length: 215 amino acids
Theoretical weight: 23.57 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P38624 (Residues: 1-215; Coverage: 100%)
Gene names: J1407, PRE3, YJL001W
Sequence domains: Proteasome subunit
Proteasome subunit beta type-2 Chains: 2, i
Molecule details ›
Chains: 2, i
Length: 261 amino acids
Theoretical weight: 28.3 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P25043 (Residues: 1-261; Coverage: 100%)
Gene names: PUP1, YOR157C
Sequence domains:
Proteasome subunit beta type-3 Chains: 3, j
Molecule details ›
Chains: 3, j
Length: 205 amino acids
Theoretical weight: 22.63 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P25451 (Residues: 1-205; Coverage: 100%)
Gene names: PUP3, YER094C
Sequence domains: Proteasome subunit
Proteasome subunit beta type-4 Chains: 4, k
Molecule details ›
Chains: 4, k
Length: 198 amino acids
Theoretical weight: 22.55 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P22141 (Residues: 1-198; Coverage: 100%)
Gene names: PRE1, YER012W
Sequence domains: Proteasome subunit
Proteasome subunit beta type-5 Chains: 5, l
Molecule details ›
Chains: 5, l
Length: 287 amino acids
Theoretical weight: 31.67 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P30656 (Residues: 1-287; Coverage: 100%)
Gene names: DOA3, P8283.10, PRE2, PRG1, YPR103W
Sequence domains: Proteasome subunit
Proteasome subunit beta type-6 Chains: 6, m
Molecule details ›
Chains: 6, m
Length: 241 amino acids
Theoretical weight: 26.91 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P23724 (Residues: 1-241; Coverage: 100%)
Gene names: PRE7, PRS3, PTS1, YBL0407, YBL041W
Sequence domains: Proteasome subunit
Proteasome subunit beta type-7 Chains: 7, n
Molecule details ›
Chains: 7, n
Length: 266 amino acids
Theoretical weight: 29.47 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P30657 (Residues: 1-266; Coverage: 100%)
Gene names: PRE4, YFR050C
Sequence domains: Proteasome subunit
26S proteasome regulatory subunit 7 homolog Chain: H
Molecule details ›
Chain: H
Length: 467 amino acids
Theoretical weight: 52.05 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P33299 (Residues: 1-467; Coverage: 100%)
Gene names: CIM5, RPT1, YKL145W, YTA3
Sequence domains:
26S proteasome regulatory subunit 4 homolog Chain: I
Molecule details ›
Chain: I
Length: 437 amino acids
Theoretical weight: 48.9 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P40327 (Residues: 1-437; Coverage: 100%)
Gene names: D2920, RPT2, YDL007W, YHS4, YTA5
Sequence domains:
26S proteasome regulatory subunit 6B homolog Chain: K
Molecule details ›
Chain: K
Length: 428 amino acids
Theoretical weight: 47.95 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P33298 (Residues: 1-428; Coverage: 100%)
Gene names: D9509.14, RPT3, YDR394W, YNT1, YTA2
Sequence domains:
26S proteasome subunit RPT4 Chain: L
Molecule details ›
Chain: L
Length: 437 amino acids
Theoretical weight: 49.48 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P53549 (Residues: 1-437; Coverage: 100%)
Gene names: CRL13, PCS1, RPT4, SUG2, YOR259C
Sequence domains:
26S proteasome regulatory subunit 6A Chain: M
Molecule details ›
Chain: M
Length: 434 amino acids
Theoretical weight: 48.32 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P33297 (Residues: 1-434; Coverage: 100%)
Gene names: O3258, RPT5, YOR117W, YOR3258W, YTA1
Sequence domains:
26S proteasome regulatory subunit 8 homolog Chain: J
Molecule details ›
Chain: J
Length: 405 amino acids
Theoretical weight: 45.34 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: Q01939 (Residues: 1-405; Coverage: 100%)
Gene names: CIM3, CRL3, RPT6, SUG1, TBPY, TBY1, YGL048C
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand MG 6 x MG
Ligand ATP 5 x ATP
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.5Å
Relevant EMDB volumes: EMD-3535

Quick links

Citations

28 review citations

The Logic of the 26S Proteasome.
Collins et al. (2017)
27 more

20 mentions without citation

Fibrous scaffolds for building hearts and heart parts.
Capulli et al. (2016)
19 more