› 5mpf

X-ray diffraction
2.92Å resolution

Structural Basis of Gene Regulation by the Grainyhead Transcription Factor Superfamily

Released:
DOI: 10.2210/pdb5mpf/pdb
PDB entry 5mpf coloured by chain, front view.
PDB entry 5mpf coloured by chain, side view.
PDB entry 5mpf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of gene regulation by the Grainyhead/CP2 transcription factor family.
Ming Q, Roske Y, Schuetz A, Walentin K, Ibraimi I, Schmidt-Ott KM, Heinemann U
Nucleic Acids Res 46 2082-2095 (2018)
PMID: 29309642

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119032 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Grainyhead-like protein 1 homolog Chains: A, B
Molecule details ›
Chains: A, B
Length: 238 amino acids
Theoretical weight: 27.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NZI5 (Residues: 248-485; Coverage: 39%)
Gene names: GRHL1, LBP32, MGR, TFCP2L2
Sequence domains: CP2 transcription factor
DNA (5'-D(*AP*AP*AP*AP*CP*CP*GP*GP*TP*TP*TP*T)-3') Chains: E, F
Molecule details ›
Chains: E, F
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P41
Unit cell:
a: 59.034Å b: 59.034Å c: 198.854Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.228 0.276
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

7 review citations

Grainyhead-like transcription factors in cancer - Focus on recent developments.
Kotarba et al. (2020)
6 more

1 mention without citation

Structural basis of gene regulation by the Grainyhead/CP2 transcription factor family.
Ming et al. (2018)