› 5mpt

X-ray diffraction
1.65Å resolution

Structure of the citrinin polyketide synthase CMeT domain

Released:
DOI: 10.2210/pdb5mpt/pdb
PDB entry 5mpt coloured by chain, front view.
PDB entry 5mpt coloured by chain, side view.
PDB entry 5mpt coloured by chain, top view.
Source organism: Monascus purpureus
Primary publication:
Functional and Structural Analysis of Programmed C-Methylation in the Biosynthesis of the Fungal Polyketide Citrinin.
Storm PA, Herbst DA, Maier T, Townsend CA
Cell Chem Biol 24 316-325 (2017)
PMID: 28238725

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179236 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Citrinin polyketide synthase Chain: A
Molecule details ›
Chain: A
Length: 414 amino acids
Theoretical weight: 46.76 KDa
Source organism: Monascus purpureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q65Z23 (Residues: 1780-2182; Coverage: 16%)
Gene name: pksCT
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand SAH 1 x SAH
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: R3
Unit cell:
a: 98.37Å b: 98.37Å c: 133.43Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.187 0.217
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Classic fungal natural products in the genomic age: the molecular legacy of Harold Raistrick.
Schor et al. (2018)
1 more

3 mentions without citation

Functional and Structural Analysis of Programmed C-Methylation in the Biosynthesis of the Fungal Polyketide Citrinin.
Storm et al. (2017)
2 more