› 5msg

X-ray diffraction
3.8Å resolution

Influenza B polymerase bound to vRNA promoter and capped RNA primer

Released:
DOI: 10.2210/pdb5msg/pdb
PDB entry 5msg coloured by chain, front view.
PDB entry 5msg coloured by chain, side view.
PDB entry 5msg coloured by chain, top view.
Source organism: Influenza B virus
Primary publication:
An in vitro fluorescence based study of initiation of RNA synthesis by influenza B polymerase.
Reich S, Guilligay D, Cusack S
Nucleic Acids Res 45 3353-3368 (2017)
PMID: 28126917

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-178410 (preferred)
Entry contents:
3 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (6 distinct):
Polymerase acidic protein Chain: A
Molecule details ›
Chain: A
Length: 751 amino acids
Theoretical weight: 85.82 KDa
Source organism: Influenza B virus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q5V8Z9 (Residues: 1-726; Coverage: 100%)
Gene name: PA
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PA
RNA-directed RNA polymerase catalytic subunit Chain: B
Molecule details ›
Chain: B
Length: 772 amino acids
Theoretical weight: 86.21 KDa
Source organism: Influenza B virus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q5V8Y6 (Residues: 1-752; Coverage: 100%)
Gene name: PB1
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PB1
Polymerase basic protein 2 Chain: C
Molecule details ›
Chain: C
Length: 798 amino acids
Theoretical weight: 90.84 KDa
Source organism: Influenza B virus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q5V8X3 (Residues: 1-770; Coverage: 100%)
Gene name: PB2
Sequence domains:
RNA (5'-D(*(GDM))-R(P*AP*AP*UP*C)-3') Chain: M
Molecule details ›
Chain: M
Length: 14 nucleotides
Theoretical weight: 4.55 KDa
RNA (5'-R(*UP*AP*UP*AP*CP*CP*UP*CP*UP*GP*CP*UP*UP*CP*UP*GP*CP*U)-3') Chain: R
Molecule details ›
Chain: R
Length: 18 nucleotides
Theoretical weight: 5.58 KDa
RNA (5'-R(P*AP*GP*UP*AP*GP*UP*AP*AP*CP*AP*AP*GP*AP*G)-3') Chain: V
Molecule details ›
Chain: V
Length: 14 nucleotides
Theoretical weight: 4.56 KDa

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P3221
Unit cell:
a: 200.41Å b: 200.41Å c: 254.61Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.236 0.235 0.267
Expression system: Trichoplusia ni

Quick links

Citations

8 review citations

Influenza A Virus Cell Entry, Replication, Virion Assembly and Movement.
Dou et al. (2018)
7 more

12 mentions without citation

Interplay between Influenza Virus and the Host RNA Polymerase II Transcriptional Machinery.
Walker et al. (2019)
11 more