› 5mtm

X-ray diffraction
2.4Å resolution

Monobody Mb(Lck_3) bound to Lck-SH2 domain

Released:
DOI: 10.2210/pdb5mtm/pdb
PDB entry 5mtm coloured by chain, front view.
PDB entry 5mtm coloured by chain, side view.
PDB entry 5mtm coloured by chain, top view.
Source organisms:
Primary publication:
Selective Targeting of SH2 Domain-Phosphotyrosine Interactions of Src Family Tyrosine Kinases with Monobodies.
Kükenshöner T, Schmit NE, Bouda E, Sha F, Pojer F, Koide A, Seeliger M, Koide S, Hantschel O
J Mol Biol 429 1364-1380 (2017)
PMID: 28347651

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138954 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein kinase Lck Chain: A
Molecule details ›
Chain: A
Length: 116 amino acids
Theoretical weight: 13.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06239 (Residues: 118-231; Coverage: 22%)
Gene name: LCK
Sequence domains: SH2 domain
Structure domains: SH2 domain
Monobody Mb(Lck_3) Chain: B
Molecule details ›
Chain: B
Length: 95 amino acids
Theoretical weight: 10.14 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P41212
Unit cell:
a: 81.834Å b: 81.834Å c: 105.961Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.205 0.257
Expression system: Escherichia coli

Quick links

Citations

5 review citations

SOCS Proteins in Immunity, Inflammatory Diseases, and Immune-Related Cancer.
Sobah et al. (2021)
4 more

3 mentions without citation

Monobodies as enabling tools for structural and mechanistic biology.
Hantschel et al. (2020)
2 more