5mv8

X-ray diffraction
1.88Å resolution

Structure of human Myosin 7b C-terminal MyTH4-FERM domain in complex with harmonin-a PDZ3 domain

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-180168 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Unconventional myosin-VIIb Chain: A
Molecule details ›
Chain: A
Length: 527 amino acids
Theoretical weight: 60.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6PIF6 (Residues: 1609-2116; Coverage: 24%)
Gene name: MYO7B
Sequence domains:
Harmonin Chain: B
Molecule details ›
Chain: B
Length: 130 amino acids
Theoretical weight: 14.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y6N9 (Residues: 428-552; Coverage: 23%)
Gene names: AIE75, USH1C
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P21
Unit cell:
a: 69.62Å b: 42.56Å c: 118.17Å
α: 90° β: 98.02° γ: 90°
R-values:
R R work R free
0.191 0.19 0.219
Expression system: Escherichia coli