› 5mwc

X-ray diffraction
2.45Å resolution

Crystal structure of the genetically-encoded green calcium indicator NTnC in its calcium bound state

Released:
DOI: 10.2210/pdb5mwc/pdb
PDB entry 5mwc coloured by chain, front view.
PDB entry 5mwc coloured by chain, side view.
PDB entry 5mwc coloured by chain, top view.
Source organism: synthetic construct
Entry authors: Boyko KM, Nikolaeva AY, Korzhenevskiy DA, Rakitina TV, Popov VO, Subach OM, Barykina NV, Subach FV

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166010 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
genetically-encoded green calcium indicator NTnC Chains: A, D
Molecule details ›
Chains: A, D
Length: 309 amino acids
Theoretical weight: 35.05 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand CA 3 x CA
1 modified residue:
Ligand CR2 2 x CR2

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: P41212
Unit cell:
a: 82.1Å b: 82.1Å c: 157.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.239 0.31
Expression system: Escherichia coli

Quick links

Citations

5 mentions without citation

Novel Genetically Encoded Bright Positive Calcium Indicator NCaMP7 Based on the mNeonGreen Fluorescent Protein.
Subach et al. (2020)
4 more