› 5mxp

X-ray diffraction
1.45Å resolution

Haloalkane dehalogenase DmxA from Marinobacter sp. ELB17 possessing a unique catalytic residue

Released:
DOI: 10.2210/pdb5mxp/pdb
Model geometry
Fit model/data
PDB entry 5mxp coloured by chain, front view.
PDB entry 5mxp coloured by chain, side view.
PDB entry 5mxp coloured by chain, top view.
Source organism: Marinobacter sp. ELB17
Primary publication:
Deciphering the Structural Basis of High Thermostability of Dehalogenase from Psychrophilic Bacterium Marinobacter sp. ELB17.
Chrast L, Tratsiak K, Planas-Iglesias J, Daniel L, Prudnikova T, Brezovsky J, Bednar D, Kuta Smatanova I, Chaloupkova R, Damborsky J
OpenAccess logo Microorganisms 7 (2019)
PMID: 31661858

Function and Biology Details

Reaction catalysed: 
1-haloalkane + H(2)O = a primary alcohol + halide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107154 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 302 amino acids
Theoretical weight: 34.92 KDa
Source organism: Marinobacter sp. ELB17
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A3JB27 (Residues: 1-296; Coverage: 100%)
Gene name: MELB17_23105
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand ACT 3 x ACT
Ligand NA 8 x NA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 43.371Å b: 78.343Å c: 150.514Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.173 0.214
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Power of Biocatalysis for Organic Synthesis.
Winkler et al. (2021)
1 more

2 mentions without citation

Deciphering the Structural Basis of High Thermostability of Dehalogenase from Psychrophilic Bacterium Marinobacter sp. ELB17.
Chrast et al. (2019)
1 more