› 5mze

X-ray diffraction
2.1Å resolution

Crystal structure of mouse MTH1 with 8-oxo-dGTP

Released:
DOI: 10.2210/pdb5mze/pdb
Model geometry
Fit model/data
PDB entry 5mze coloured by chain, front view.
PDB entry 5mze coloured by chain, side view.
PDB entry 5mze coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal Structures and Inhibitor Interactions of Mouse and Dog MTH1 Reveal Species-Specific Differences in Affinity.
Narwal M, Jemth AS, Gustafsson R, Almlöf I, Warpman Berglund U, Helleday T, Stenmark P
Biochemistry 57 593-603 (2018)
PMID: 29281266

Function and Biology Details

Reaction catalysed: 
2-hydroxy-dATP + H(2)O = 2-hydroxy-dAMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156794 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oxidized purine nucleoside triphosphate hydrolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 176 amino acids
Theoretical weight: 20.1 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P53368 (Residues: 1-156; Coverage: 100%)
Gene names: Mth1, Nudt1
Sequence domains: NUDIX domain
Structure domains: Nucleoside Triphosphate Pyrophosphohydrolase

Ligands and Environments

4 bound ligands:
Ligand CU 4 x CU
Ligand PEG 2 x PEG
Ligand 8DG 4 x 8DG
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 58.532Å b: 139.245Å c: 58.718Å
α: 90° β: 107.53° γ: 90°
R-values:
R R work R free
0.203 0.201 0.239
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

The MTH1 inhibitor TH588 is a microtubule-modulating agent that eliminates cancer cells by activating the mitotic surveillance pathway.
Gul et al. (2019)
2 more

1 mention without citation

Structural Insight into Molecular Inhibitory Mechanism of InsP6 on African Swine Fever Virus mRNA-Decapping Enzyme g5Rp.
Yang et al. (2022)