› 5n2m

X-ray diffraction
1.54Å resolution

Crystal structure of the first bromodomain of human BRD4 in complex with a tetrahydroquinoline analogue

Released:
DOI: 10.2210/pdb5n2m/pdb
PDB entry 5n2m coloured by chain, front view.
PDB entry 5n2m coloured by chain, side view.
PDB entry 5n2m coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Tallant C, Slavish PJ, Siejka P, Bharatham N, Shadrick WR, Chai S, Young BM, Boyd VA, Heroven C, Wiggers HJ, Picaud S, Fedorov O, Krojer T, Chen T, Lee RE, Guy RK, Shelat AA, von Delft F, Arrowsmith CH, Edwards AM, Bountra C, Knapp S, Structural Genomics Consortium (SGC)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130112 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bromodomain-containing protein 4 Chain: A
Molecule details ›
Chain: A
Length: 127 amino acids
Theoretical weight: 15.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O60885 (Residues: 44-168; Coverage: 9%)
Gene names: BRD4, HUNK1
Sequence domains: Bromodomain
Structure domains: Bromodomain-like

Ligands and Environments

2 bound ligands:
Ligand 8J2 1 x 8J2
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 38.797Å b: 42.09Å c: 92.576Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.23
Expression system: Escherichia coli

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