› 5n6m

X-ray diffraction
3.1Å resolution

Structure of the membrane integral lipoprotein N-acyltransferase Lnt from P. aeruginosa

Released:
DOI: 10.2210/pdb5n6m/pdb
PDB entry 5n6m coloured by chain, front view.
PDB entry 5n6m coloured by chain, side view.
PDB entry 5n6m coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structural insights into the mechanism of the membrane integral N-acyltransferase step in bacterial lipoprotein synthesis.
Wiktor M, Weichert D, Howe N, Huang CY, Olieric V, Boland C, Bailey J, Vogeley L, Stansfeld PJ, Buddelmeijer N, Wang M, Caffrey M
Nat Commun 8 15952 (2017)
PMID: 28675161

Function and Biology Details

Reaction catalysed: 
A phosphoglycerolipid + an [apolipoprotein]-S-1,2-diacyl-sn-glyceryl-L-cysteine = a 1-lyso-phosphoglycerolipid + a [lipoprotein]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195658 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Apolipoprotein N-acyltransferase Chain: A
Molecule details ›
Chain: A
Length: 531 amino acids
Theoretical weight: 58.23 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Pseudomonas aeruginosa
UniProt:
  • Canonical: Q9ZI86 (Residues: 1-511; Coverage: 100%)
Gene names: PA3984, cutE, lnt
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand GOL 3 x GOL
Ligand FLC 1 x FLC
Ligand OLC 5 x OLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 152.73Å b: 39.23Å c: 102.25Å
α: 90° β: 116.26° γ: 90°
R-values:
R R work R free
0.23 0.225 0.273
Expression system: Pseudomonas aeruginosa

Quick links

Citations

5 review citations

Structure and Mechanism of DHHC Protein Acyltransferases.
Stix et al. (2020)
4 more

2 mentions without citation

Structural insights into the mechanism of the membrane integral N-acyltransferase step in bacterial lipoprotein synthesis.
Wiktor et al. (2017)
1 more