› 5n6o

X-ray diffraction
2.59Å resolution

Wild type human Rac1-GDP

Released:
DOI: 10.2210/pdb5n6o/pdb
PDB entry 5n6o coloured by chain, front view.
PDB entry 5n6o coloured by chain, side view.
PDB entry 5n6o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Allosteric inhibition of the guanine nucleotide exchange factor DOCK5 by a small molecule.
Ferrandez Y, Zhang W, Peurois F, Akendengu�� L, Blangy A, Zeghouf M, Cherfils J
Sci Rep 7 14409 (2017)
PMID: 29089502

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158894 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related C3 botulinum toxin substrate 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 176 amino acids
Theoretical weight: 19.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P63000 (Residues: 2-177; Coverage: 92%)
Gene names: MIG5, RAC1, TC25
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GDP 2 x GDP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P212121
Unit cell:
a: 41.641Å b: 82.656Å c: 105.898Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.193 0.252
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

3 review citations

Targeting the Small GTPase Superfamily through Their Regulatory Proteins.
Gray et al. (2020)
2 more

9 mentions without citation

Allosteric inhibition of the guanine nucleotide exchange factor DOCK5 by a small molecule.
Ferrandez et al. (2017)
8 more