› 5n7d

X-ray diffraction
2.3Å resolution

MAGI-1 complexed with a RSK1 peptide

Released:
DOI: 10.2210/pdb5n7d/pdb
PDB entry 5n7d coloured by chain, front view.
PDB entry 5n7d coloured by chain, side view.
PDB entry 5n7d coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Dynamic control of RSK complexes by phosphoswitch-based regulation.
Gógl G, Biri-Kovács B, Póti ÁL, Vadászi H, Szeder B, Bodor A, Schlosser G, Ács A, Turiák L, Buday L, Alexa A, Nyitray L, Reményi A
FEBS J 285 46-71 (2018)
PMID: 29083550

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-139546 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Annexin A2; Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 427 amino acids
Theoretical weight: 48.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96QZ7 (Residues: 455-558; Coverage: 7%)
  • Canonical: P07355 (Residues: 22-339; Coverage: 94%)
Gene names: AIP3, ANX2, ANX2L4, ANXA2, BAIAP1, BAP1, CAL1H, LPC2D, MAGI1, TNRC19
Sequence domains:
Ribosomal protein S6 kinase alpha-1 Chain: C
Molecule details ›
Chain: C
Length: 49 amino acids
Theoretical weight: 5.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15418 (Residues: 688-735; Coverage: 7%)
Gene names: MAPKAPK1A, RPS6KA1, RSK1

Ligands and Environments

2 bound ligands:
Ligand GOL 5 x GOL
Ligand CA 7 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: C2
Unit cell:
a: 195.81Å b: 60.68Å c: 99.71Å
α: 90° β: 98.81° γ: 90°
R-values:
R R work R free
0.192 0.191 0.227
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Structural Features of Tight-Junction Proteins.
Heinemann et al. (2019)
4 more

2 mentions without citation

Dynamic control of RSK complexes by phosphoswitch-based regulation.
Gógl et al. (2018)
1 more