› 5n8t

X-ray diffraction
1.61Å resolution

CRYSTAL STRUCTURE OF STREPTAVIDIN D-amino acid containing peptide Gdlwqheatwkkq

Released:
DOI: 10.2210/pdb5n8t/pdb
PDB entry 5n8t coloured by chain, front view.
PDB entry 5n8t coloured by chain, side view.
PDB entry 5n8t coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Stepwise Evolution Improves Identification of Diverse Peptides Binding to a Protein Target.
Lyamichev VI, Goodrich LE, Sullivan EH, Bannen RM, Benz J, Albert TJ, Patel JJ
Sci Rep 7 12116 (2017)
PMID: 28935886

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149733 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chain: A
Molecule details ›
Chain: A
Length: 183 amino acids
Theoretical weight: 18.85 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli
UniProt:
  • Canonical: P22629 (Residues: 1-183; Coverage: 100%)
Sequence domains: Avidin family
Structure domains: Avidin-like
DLE-DTR-DGN-DHI-DGL-DAL-DTH-DTR-DLY Chain: B
Molecule details ›
Chain: B
Length: 9 amino acids
Theoretical weight: 1.2 KDa
Source organism: Streptomyces avidinii
Expression system: Not provided

Ligands and Environments

No bound ligands
8 modified residues:
Ligand DLE 1 x DLE
Ligand DTR 2 x DTR
Ligand DGN 1 x DGN
Ligand DHI 1 x DHI
Ligand DGL 1 x DGL
Ligand DAL 1 x DAL
Ligand DTH 1 x DTH
Ligand DLY 1 x DLY

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P42212
Unit cell:
a: 57.779Å b: 57.779Å c: 84.405Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.243
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

New Immunoinformatics Tools for Swine: Designing Epitope-Driven Vaccines, Predicting Vaccine Efficacy, and Making Vaccines on Demand.
Moise et al. (2020)
1 more

1 mention without citation

Prediction of Protein Complex Structure Using Surface-Induced Dissociation and Cryo-Electron Microscopy.
Seffernick et al. (2021)