› 5n9o

X-ray diffraction
1.53Å resolution

EGFP(enhanced green fluorescent protein) mutant - L232H

Released:
DOI: 10.2210/pdb5n9o/pdb
PDB entry 5n9o coloured by chain, front view.
PDB entry 5n9o coloured by chain, side view.
PDB entry 5n9o coloured by chain, top view.
Source organism: Aequorea victoria
Entry authors: Kachalova GS, Popov AP, Simanovskaya AA, Krukova MV, Lipkin AV

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 26.98 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand AE4 2 x AE4
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 84.595Å b: 67.931Å c: 47.266Å
α: 90° β: 118.66° γ: 90°
R-values:
R R work R free
0.169 0.167 0.211
Expression system: Escherichia coli BL21(DE3)

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