› 5na4

X-ray diffraction
2.55Å resolution

NADH:quinone oxidoreductase (NDH-II) from Staphylococcus aureus - E172S mutant

Released:
DOI: 10.2210/pdb5na4/pdb
PDB entry 5na4 coloured by chain, front view.
PDB entry 5na4 coloured by chain, side view.
PDB entry 5na4 coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
The key role of glutamate 172 in the mechanism of type II NADH:quinone oxidoreductase of Staphylococcus aureus.
Sousa FM, Sena FV, Batista AP, Athayde D, Brito JA, Archer M, Oliveira ASF, Soares CM, Catarino T, Pereira MM
Biochim Biophys Acta Bioenerg 1858 823-832 (2017)
PMID: 28801048

Function and Biology Details

Reaction catalysed: 
NADH + a quinone = NAD(+) + a quinol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173489 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II NADH:quinone oxidoreductase Chain: A
Molecule details ›
Chain: A
Length: 408 amino acids
Theoretical weight: 44.95 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2FZV7 (Residues: 1-402; Coverage: 100%)
Gene name: SAOUHSC_00878
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: P4332
Unit cell:
a: 151.275Å b: 151.275Å c: 151.275Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.175 0.222
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Architecture of bacterial respiratory chains.
Kaila et al. (2021)
1 more

1 mention without citation

Inhibition of planktonic growth and biofilm formation of Staphylococcus aureus by entrectinib through disrupting the cell membrane.
Liu et al. (2022)