› 5nc5

X-ray diffraction
3.2Å resolution

Crystal structure of AcrBZ in complex with antibiotic puromycin

Released:
DOI: 10.2210/pdb5nc5/pdb
PDB entry 5nc5 coloured by chain, front view.
PDB entry 5nc5 coloured by chain, side view.
PDB entry 5nc5 coloured by chain, top view.
Source organisms:
Primary publication:
An allosteric transport mechanism for the AcrAB-TolC multidrug efflux pump.
Wang Z, Fan G, Hryc CF, Blaza JN, Serysheva II, Schmid MF, Chiu W, Luisi BF, Du D
Elife 6 (2017)
PMID: 28355133

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-142007 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Multidrug efflux pump subunit AcrB Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 1049 amino acids
Theoretical weight: 113.67 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P31224 (Residues: 1-1049; Coverage: 100%)
Gene names: JW0451, acrB, acrE, b0462
Sequence domains: AcrB/AcrD/AcrF family
Structure domains:
DARPin Chains: D, E
Molecule details ›
Chains: D, E
Length: 169 amino acids
Theoretical weight: 18.32 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Structure domains: Ankyrin repeat-containing domain
Multidrug efflux pump accessory protein AcrZ Chains: F, G, H
Molecule details ›
Chains: F, G, H
Length: 49 amino acids
Theoretical weight: 5.3 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AAX1 (Residues: 1-49; Coverage: 100%)
Gene names: ECs0790, Z0932, acrZ
Sequence domains: Multidrug efflux pump-associated protein AcrZ

Ligands and Environments

6 bound ligands:
Ligand LMT 3 x LMT
Ligand D12 8 x D12
Ligand D10 26 x D10
Ligand DD9 14 x DD9
Ligand PUY 1 x PUY
Ligand HEX 1 x HEX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P212121
Unit cell:
a: 147.245Å b: 167.646Å c: 249.98Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.183 0.245
Expression system: Escherichia coli

Quick links

Citations

31 review citations

Multidrug efflux pumps: structure, function and regulation.
Du et al. (2018)
30 more

8 mentions without citation

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
Alav et al. (2021)
7 more