› 5nc7

X-ray diffraction
2.7Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152607 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein enabled homolog Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 113 amino acids
Theoretical weight: 12.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q8N8S7 (Residues: 1-111; Coverage: 19%)
Gene names: ENAH, MENA
Sequence domains: WH1 domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)
Actin assembly-inducing protein Chains: I, J, K, L, W, X, Y, Z
Molecule details ›
Chains: I, J, K, L, W, X, Y, Z
Length: 12 amino acids
Theoretical weight: 1.2 KDa
Source organism: Listeria monocytogenes
Expression system: Not provided
UniProt:
  • Canonical: P33379 (Residues: 335-344; Coverage: 2%)
Gene names: actA, lmo0204, prtB

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: C2
Unit cell:
a: 187.96Å b: 34.43Å c: 110.99Å
α: 90° β: 91.53° γ: 90°
R-values:
R R work R free
0.212 0.209 0.272
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Not provided

Quick links

Citations

4 review citations

Migration and homeostasis of regulatory T cells in rheumatoid arthritis.
Kotschenreuther et al. (2022)
3 more

2 mentions without citation

Native proline-rich motifs exploit sequence context to target actin-remodeling Ena/VASP protein ENAH.
Hwang et al. (2022)
1 more