› 5nd5

X-ray diffraction
1.74Å resolution

Crystal structure of transketolase from Chlamydomonas reinhardtii in complex with TPP and Mg2+

Released:
DOI: 10.2210/pdb5nd5/pdb
PDB entry 5nd5 coloured by chain, front view.
PDB entry 5nd5 coloured by chain, side view.
PDB entry 5nd5 coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
Structural basis for the magnesium-dependent activation of transketolase from Chlamydomonas reinhardtii.
Pasquini M, Fermani S, Tedesco D, Sciabolini C, Crozet P, Naldi M, Henri J, Vothknecht U, Bertucci C, Lemaire SD, Zaffagnini M, Francia F
Biochim Biophys Acta Gen Subj 1861 2132-2145 (2017)
PMID: 28552632

Function and Biology Details

Reaction catalysed: 
Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108662 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
transketolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 692 amino acids
Theoretical weight: 75.27 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli
UniProt:
  • Canonical: A8IAN1 (Residues: 36-718; Coverage: 95%)
Gene name: CHLRE_02g080200v5
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand TPP 2 x TPP
1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: C2
Unit cell:
a: 201.489Å b: 75.93Å c: 103.759Å
α: 90° β: 109.99° γ: 90°
R-values:
R R work R free
0.147 0.145 0.186
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Crystal Structure of Chloroplastic Thioredoxin f2 from Chlamydomonas reinhardtii Reveals Distinct Surface Properties.
Lemaire et al. (2018)
2 more