› 5ne4

X-ray diffraction
2.3Å resolution

Crystal Structure of Foot and Mouth Disease Virus O PanAsia

Released:
DOI: 10.2210/pdb5ne4/pdb
PDB entry 5ne4 coloured by chain, front view.
PDB entry 5ne4 coloured by chain, side view.
PDB entry 5ne4 coloured by chain, top view.
Source organisms:
Primary publication:
Rules of engagement between αvβ6 integrin and foot-and-mouth disease virus.
Kotecha A, Wang Q, Dong X, Ilca SL, Ondiviela M, Zihe R, Seago J, Charleston B, Fry EE, Abrescia NGA, Springer TA, Huiskonen JT, Stuart DI
Nat Commun 8 15408 (2017)
PMID: 28534487

Function and Biology Details

Reactions catalysed: 
Autocatalytically cleaves itself from the polyprotein of the foot-and-mouth disease virus by hydrolysis of a Lys-|-Gly bond, but then cleaves host cell initiation factor eIF-4G at bonds -Gly-|-Arg- and -Lys-|-Arg-.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 240-mer (preferred)
PDBe Complex ID:
PDB-CPX-180620 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Viral protein 1 (VP1) Chain: 1
Molecule details ›
Chain: 1
Length: 211 amino acids
Theoretical weight: 23.34 KDa
Source organism: Foot-and-mouth disease virus
Expression system: Cricetinae
UniProt:
  • Canonical: Q98W00 (Residues: 1-211; Coverage: 99%)
Gene name: 1D
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
O PanAsia VP2 Chain: 2
Molecule details ›
Chain: 2
Length: 218 amino acids
Theoretical weight: 24.39 KDa
Source organism: Foot-and-mouth disease virus
Expression system: Cricetinae
UniProt:
  • Canonical: Q6ZZ87 (Residues: 287-504; Coverage: 9%)
Gene name: polyprotein
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
Polyprotein Chain: 3
Molecule details ›
Chain: 3
Length: 220 amino acids
Theoretical weight: 23.94 KDa
Source organism: Foot-and-mouth disease virus O
Expression system: Cricetinae
UniProt:
  • Canonical: Q7TD07 (Residues: 505-724; Coverage: 9%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
O PanAsia VP4 Chain: 4
Molecule details ›
Chain: 4
Length: 85 amino acids
Theoretical weight: 8.79 KDa
Source organism: Foot-and-mouth disease virus
Expression system: Cricetinae
UniProt:
  • Canonical: Q7TD07 (Residues: 202-286; Coverage: 4%)
Sequence domains: Viral protein VP4 subunit
Structure domains: Capsid protein VP4 superfamily, Picornavirus

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: I23
Unit cell:
a: 345.01Å b: 345.01Å c: 345.01Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.22
Expression system: Cricetinae

Quick links

Citations

14 review citations

Virus-Receptor Interactions: The Key to Cellular Invasion.
Maginnis MS. (2018)
13 more

4 mentions without citation

Rules of engagement between αvβ6 integrin and foot-and-mouth disease virus.
Kotecha et al. (2017)
3 more