› 5nfs

X-ray diffraction
1.8Å resolution

Structure of coxsackievirus B3 3C protease in complex with the alpha-ketoamide (S)-N-benzyl-3-((S)-2-cinnamamido-3-phenylpropanamido)-2-oxo-4-((S)-2-oxopyrrolidin-3-yl)butanamide (cinnamoyl-phenylalanine-GlnLactam-CO-CO-NH-benzyl)

Released:
DOI: 10.2210/pdb5nfs/pdb
PDB entry 5nfs coloured by chain, front view.
PDB entry 5nfs coloured by chain, side view.
PDB entry 5nfs coloured by chain, top view.
Source organism: Coxsackievirus B3
Entry authors: Ma Q, Zhang L, Hilgenfeld R

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178362 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Genome polyprotein Chain: A
Molecule details ›
Chain: A
Length: 184 amino acids
Theoretical weight: 20.43 KDa
Source organism: Coxsackievirus B3
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5UEA2 (Residues: 1540-1723; Coverage: 8%)
Sequence domains: 3C cysteine protease (picornain 3C)
Structure domains: Trypsin-like serine proteases

Ligands and Environments

1 bound ligand:
Ligand D03 1 x D03
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: C2
Unit cell:
a: 77.657Å b: 64.354Å c: 39.477Å
α: 90° β: 115.44° γ: 90°
R-values:
R R work R free
0.182 0.179 0.239
Expression system: Escherichia coli

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