› 5ng7

X-ray diffraction
1.39Å resolution

Novel epoxide hydrolases belonging to the alpha/beta hydrolases superfamily in metagenomes from hot environments

Released:
DOI: 10.2210/pdb5ng7/pdb
PDB entry 5ng7 coloured by chain, front view.
PDB entry 5ng7 coloured by chain, side view.
PDB entry 5ng7 coloured by chain, top view.
Source organism: metagenome
Primary publication:
New Thermophilic α/β Class Epoxide Hydrolases Found in Metagenomes From Hot Environments.
Ferrandi EE, Sayer C, De Rose SA, Guazzelli E, Marchesi C, Saneei V, Isupov MN, Littlechild JA, Monti D
Front Bioeng Biotechnol 6 144 (2018)
PMID: 30386778

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166027 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
epoxide hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 299 amino acids
Theoretical weight: 34.94 KDa
Source organism: metagenome
Expression system: Escherichia coli
Structure domains: alpha/beta hydrolase

Ligands and Environments

4 bound ligands:
Ligand CL 3 x CL
Ligand EDO 3 x EDO
Ligand SER 1 x SER
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2
Unit cell:
a: 163.94Å b: 46.22Å c: 73.87Å
α: 90° β: 106.94° γ: 90°
R-values:
R R work R free
0.174 0.173 0.204
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Epoxide Hydrolases: Multipotential Biocatalysts.
Bučko et al. (2023)
8 other articles

4 mentions without citation

New Thermophilic α/β Class Epoxide Hydrolases Found in Metagenomes From Hot Environments.
Ferrandi et al. (2018)
3 more