› 5ngz

X-ray diffraction
2.4Å resolution

Ube2T in complex with fragment EM04

Released:
DOI: 10.2210/pdb5ngz/pdb
PDB entry 5ngz coloured by chain, front view.
PDB entry 5ngz coloured by chain, side view.
PDB entry 5ngz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Allosteric Targeting of the Fanconi Anemia Ubiquitin-Conjugating Enzyme Ube2T by Fragment Screening.
Morreale FE, Bortoluzzi A, Chaugule VK, Arkinson C, Walden H, Ciulli A
J Med Chem 60 4093-4098 (2017)
PMID: 28437106

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192476 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin-conjugating enzyme E2 T Chain: A
Molecule details ›
Chain: A
Length: 197 amino acids
Theoretical weight: 22.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NPD8 (Residues: 1-197; Coverage: 100%)
Gene names: HSPC150, PIG50, UBE2T
Sequence domains: Ubiquitin-conjugating enzyme
Structure domains: Ubiquitin Conjugating Enzyme

Ligands and Environments

1 bound ligand:
Ligand 2BG 1 x 2BG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P43212
Unit cell:
a: 55.817Å b: 55.817Å c: 185.278Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.21 0.247
Expression system: Escherichia coli

Quick links

Citations

3 review citations

A patent review of the ubiquitin ligase system: 2015-2018.
Li et al. (2018)
2 more

5 mentions without citation

Fanconi anemia pathway as a prospective target for cancer intervention.
Liu et al. (2020)
4 more