› 5nis

X-ray diffraction
3.16Å resolution

Neutral trehalase Nth1 from Saccharomyces cerevisiae

Released:
DOI: 10.2210/pdb5nis/pdb
PDB entry 5nis coloured by chain, front view.
PDB entry 5nis coloured by chain, side view.
PDB entry 5nis coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Molecular basis of the 14-3-3 protein-dependent activation of yeast neutral trehalase Nth1.
Alblova M, Smidova A, Docekal V, Vesely J, Herman P, Obsilova V, Obsil T
Proc Natl Acad Sci U S A 114 E9811-E9820 (2017)
PMID: 29087344

Function and Biology Details

Reaction catalysed: 
Alpha,alpha-trehalose + H(2)O = beta-D-glucose + alpha-D-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152273 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytosolic neutral trehalase Chain: A
Molecule details ›
Chain: A
Length: 656 amino acids
Theoretical weight: 75.07 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P32356 (Residues: 100-751; Coverage: 87%)
Gene names: NTH, NTH1, YD8119.07C, YDR001C
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P6222
Unit cell:
a: 187.308Å b: 187.308Å c: 119.973Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.198 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

14-3-3 Proteins Are on the Crossroads of Cancer, Aging, and Age-Related Neurodegenerative Disease.
Fan et al. (2019)
3 more

1 mention without citation

Molecular basis of the 14-3-3 protein-dependent activation of yeast neutral trehalase Nth1.
Alblova et al. (2017)