› 5nj2

X-ray diffraction
1.19Å resolution

Crystal structure of BlaC from Mycobacterium tuberculosis bound to phosphate

Released:
DOI: 10.2210/pdb5nj2/pdb
Model geometry
Fit model/data
PDB entry 5nj2 coloured by chain, front view.
PDB entry 5nj2 coloured by chain, side view.
PDB entry 5nj2 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Phosphate Promotes the Recovery of Mycobacterium tuberculosis β-Lactamase from Clavulanic Acid Inhibition.
Elings W, Tassoni R, van der Schoot SA, Luu W, Kynast JP, Dai L, Blok AJ, Timmer M, Florea BI, Pannu NS, Ubbink M
OpenAccess logo Biochemistry 56 6257-6267 (2017)
PMID: 29087696

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161732 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chains: A, B
Molecule details ›
Chains: A, B
Length: 274 amino acids
Theoretical weight: 29.48 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WKD3 (Residues: 43-307; Coverage: 97%)
Gene names: MTCY49.07c, Rv2068c, blaA, blaC
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

4 bound ligands:
Ligand PO4 3 x PO4
Ligand ACT 4 x ACT
Ligand ETE 1 x ETE
Ligand AE4 1 x AE4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: P1
Unit cell:
a: 39.591Å b: 41.683Å c: 76.841Å
α: 101.28° β: 90.11° γ: 90.43°
R-values:
R R work R free
0.168 0.167 0.19
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

Lobularia libyca: Phytochemical Profiling, Antioxidant and Antimicrobial Activity Using In Vitro and In Silico Studies.
Benchikha et al. (2022)
2 more

5 mentions without citation

New Conformations of Acylation Adducts of Inhibitors of β-Lactamase from Mycobacterium tuberculosis.
Tassoni et al. (2019)
4 more