› 5nl7

X-ray diffraction
2.48Å resolution

The crystal structure of the Actin Binding Domain (ABD) of alpha actinin from Entamoeba histolytica

Released:
DOI: 10.2210/pdb5nl7/pdb
Model geometry
Fit model/data
PDB entry 5nl7 coloured by chain, front view.
PDB entry 5nl7 coloured by chain, side view.
PDB entry 5nl7 coloured by chain, top view.
Source organism: Entamoeba histolytica
Primary publication:
Calcium modulates the domain flexibility and function of an α-actinin similar to the ancestral α-actinin.
Pinotsis N, Zielinska K, Babuta M, Arolas JL, Kostan J, Khan MB, Schreiner C, Salmazo A, Ciccarelli L, Puchinger M, Gkougkoulia EA, Ribeiro EA, Marlovits TC, Bhattacharya A, Djinovic-Carugo K
Proc Natl Acad Sci U S A 117 22101-22112 (2020)
PMID: 32848067

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111311 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calponin homology domain protein, putative Chains: A, B
Molecule details ›
Chains: A, B
Length: 234 amino acids
Theoretical weight: 26.43 KDa
Source organism: Entamoeba histolytica
Expression system: Escherichia coli
UniProt:
  • Canonical: C4LWU6 (Residues: 1-231; Coverage: 37%)
Gene name: EHI_199000
Sequence domains: Calponin homology (CH) domain
Structure domains: Calponin-like domain

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P3
Unit cell:
a: 146.74Å b: 146.74Å c: 33.97Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.196 0.252
Expression system: Escherichia coli

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Citations

3 review citations

Actin Bundles Dynamics and Architecture.
Rajan et al. (2023)
2 more