› 5nla

X-ray diffraction
2.7Å resolution

Crystal structure of the AraC-like transcriptional activator CuxR

Released:
DOI: 10.2210/pdb5nla/pdb
PDB entry 5nla coloured by chain, front view.
PDB entry 5nla coloured by chain, side view.
PDB entry 5nla coloured by chain, top view.
Source organism: Sinorhizobium meliloti 1021
Primary publication:
AraC-like transcriptional activator CuxR binds c-di-GMP by a PilZ-like mechanism to regulate extracellular polysaccharide production.
Schäper S, Steinchen W, Krol E, Altegoer F, Skotnicka D, Søgaard-Andersen L, Bange G, Becker A
Proc Natl Acad Sci U S A 114 E4822-E4831 (2017)
PMID: 28559336

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187946 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH araC/xylS-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 382 amino acids
Theoretical weight: 42.69 KDa
Source organism: Sinorhizobium meliloti 1021
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92WA5 (Residues: 1-382; Coverage: 100%)
Gene name: SM_b20457
Sequence domains: Helix-turn-helix domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P4322
Unit cell:
a: 80.662Å b: 80.662Å c: 125.596Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.236 0.281
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Ancestral zinc-finger bearing protein MucR in alpha-proteobacteria: A novel xenogeneic silencer?
Jiao et al. (2020)
1 more

2 mentions without citation

AraC-like transcriptional activator CuxR binds c-di-GMP by a PilZ-like mechanism to regulate extracellular polysaccharide production.
Schäper et al. (2017)
1 more