› 5nm8

X-ray diffraction
1.93Å resolution

Structure of PipY, the COG0325 family member of Synechococcus elongatus PCC7942, with PLP bound

Released:
DOI: 10.2210/pdb5nm8/pdb
PDB entry 5nm8 coloured by chain, front view.
PDB entry 5nm8 coloured by chain, side view.
PDB entry 5nm8 coloured by chain, top view.
Source organism: Synechococcus elongatus PCC 7942 = FACHB-805
Primary publication:
Studies on cyanobacterial protein PipY shed light on structure, potential functions, and vitamin B6 -dependent epilepsy.
Tremiño L, Forcada-Nadal A, Contreras A, Rubio V
FEBS Lett 591 3431-3442 (2017)
PMID: 28914444

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174264 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyridoxal phosphate homeostasis protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 228 amino acids
Theoretical weight: 25.3 KDa
Source organism: Synechococcus elongatus PCC 7942 = FACHB-805
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q31LH9 (Residues: 1-221; Coverage: 100%)
Gene name: Synpcc7942_2060
Sequence domains: Alanine racemase, N-terminal domain
Structure domains: Alanine racemase

Ligands and Environments

2 bound ligands:
Ligand PLP 2 x PLP
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 46.627Å b: 98.937Å c: 103.737Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.238
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

The PII-NAGK-PipX-NtcA Regulatory Axis of Cyanobacteria: A Tale of Changing Partners, Allosteric Effectors and Non-covalent Interactions.
Forcada-Nadal et al. (2018)
2 more

5 mentions without citation

Distinctive Features of PipX, a Unique Signaling Protein of Cyanobacteria.
Labella et al. (2020)
4 more