› 5nnp

X-ray diffraction
2.6Å resolution

Structure of Naa15/Naa10 bound to HypK-THB

Released:
DOI: 10.2210/pdb5nnp/pdb
PDB entry 5nnp coloured by chain, front view.
PDB entry 5nnp coloured by chain, side view.
PDB entry 5nnp coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of HypK regulating N-terminal acetylation by the NatA complex.
Weyer FA, Gumiero A, Lapouge K, Bange G, Kopp J, Sinning I
Nat Commun 8 15726 (2017)
PMID: 28585574

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-123990 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
N-terminal acetyltransferase-like protein Chains: A, E
Molecule details ›
Chains: A, E
Length: 745 amino acids
Theoretical weight: 84.91 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Escherichia coli
UniProt:
  • Canonical: G0S4M4 (Residues: 2-744; Coverage: 100%)
Gene name: CTHT_0031530
Sequence domains:
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
N-acetyltransferase domain-containing protein Chains: B, F
Molecule details ›
Chains: B, F
Length: 195 amino acids
Theoretical weight: 22.44 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Escherichia coli
UniProt:
  • Canonical: G0SEE8 (Residues: 2-189; Coverage: 77%)
Gene name: CTHT_0063490
Sequence domains: Acetyltransferase (GNAT) family
Structure domains: Aminopeptidase
Nascent polypeptide-associated complex subunit alpha-like UBA domain-containing protein Chains: C, G
Molecule details ›
Chains: C, G
Length: 62 amino acids
Theoretical weight: 6.59 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Escherichia coli
UniProt:
  • Canonical: G0SCY6 (Residues: 83-126; Coverage: 35%)
Gene name: CTHT_0058830
Sequence domains: HYPK UBA domain
Ser-Glu-Ser-Ser Chains: I, L
Molecule details ›
Chains: I, L
Length: 4 amino acids
Theoretical weight: 408 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand GOL 27 x GOL
Ligand PO4 4 x PO4
Ligand CMC 2 x CMC
1 modified residue:
Ligand FME 2 x FME

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 93.319Å b: 106.041Å c: 130.937Å
α: 90° β: 94.98° γ: 90°
R-values:
R R work R free
0.164 0.161 0.22
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

Co-translational, Post-translational, and Non-catalytic Roles of N-Terminal Acetyltransferases.
Aksnes et al. (2019)
3 more

4 mentions without citation

Structural basis of HypK regulating N-terminal acetylation by the NatA complex.
Weyer et al. (2017)
3 more