› 5nr2

X-ray diffraction
2.78Å resolution

Crystal structure of the ferric enterobactin receptor (PfeA) from Pseudomonas aeruginosa in complex with azotochelin

Released:
DOI: 10.2210/pdb5nr2/pdb
PDB entry 5nr2 coloured by chain, front view.
PDB entry 5nr2 coloured by chain, side view.
PDB entry 5nr2 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
The complex of ferric-enterobactin with its transporter from Pseudomonas aeruginosa suggests a two-site model.
Moynié L, Milenkovic S, Mislin GLA, Gasser V, Malloci G, Baco E, McCaughan RP, Page MGP, Schalk IJ, Ceccarelli M, Naismith JH
Nat Commun 10 3673 (2019)
PMID: 31413254

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170134 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferric enterobactin receptor Chain: A
Molecule details ›
Chain: A
Length: 724 amino acids
Theoretical weight: 78.74 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05098 (Residues: 26-746; Coverage: 100%)
Gene names: PA2688, pfeA
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand FE 1 x FE
Ligand 95B 1 x 95B
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21212
Unit cell:
a: 86.64Å b: 158.08Å c: 78.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.26
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Iron Acquisition Systems of Gram-negative Bacterial Pathogens Define TonB-Dependent Pathways to Novel Antibiotics.
Klebba et al. (2021)
7 more

2 mentions without citation

Genetic and structural determinants on iron assimilation pathways in the plant pathogen Xanthomonas citri subsp. citri and Xanthomonas sp.
Guerra et al. (2020)
1 more