› 5ns3

X-ray diffraction
2.4Å resolution

Crystal structures of Cy5 cyanine fluorophores stacked onto the end of double-stranded RNA

Released:
DOI: 10.2210/pdb5ns3/pdb
PDB entry 5ns3 coloured by chain, front view.
PDB entry 5ns3 coloured by chain, side view.
PDB entry 5ns3 coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Crystal Structures of Cyanine Fluorophores Stacked onto the End of Double-Stranded RNA.
Liu Y, Lilley DMJ
Biophys J 113 2336-2343 (2017)
PMID: 29211987

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154301 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Large ribosomal subunit protein uL5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 177 amino acids
Theoretical weight: 20.48 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P41201 (Residues: 5-181; Coverage: 97%)
Gene names: rpl5, rplE
Sequence domains:
double-stranded RNA Chains: C, D
Molecule details ›
Chains: C, D
Length: 34 nucleotides
Theoretical weight: 11 KDa
Source organism: Thermus thermophilus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21212
Unit cell:
a: 95.043Å b: 123.895Å c: 51.201Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.253 0.252 0.274
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices.
Lerner et al. (2021)
3 more

1 mention without citation

Crystal Structures of Cyanine Fluorophores Stacked onto the End of Double-Stranded RNA.
Liu et al. (2017)