› 5nsf

X-ray diffraction
2.43Å resolution

Function and Biology Details

Reaction catalysed: 
ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176482 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine--tRNA ligase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 318 amino acids
Theoretical weight: 36.37 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57834 (Residues: 1-306; Coverage: 100%)
Gene names: MJ0389, tyrS
Sequence domains: tRNA synthetases class I (W and Y)
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand 96Z 4 x 96Z
Ligand GOL 2 x GOL
Ligand CA 3 x CA
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P1
Unit cell:
a: 56.14Å b: 70.633Å c: 77.624Å
α: 85.7° β: 89.75° γ: 83.42°
R-values:
R R work R free
0.185 0.182 0.24
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Vibrational Spectroscopic Map, Vibrational Spectroscopy, and Intermolecular Interaction.
Baiz et al. (2020)
4 more

1 mention without citation

Orthogonal translation with 5-cyanotryptophan as an infrared probe for local structural information, electrostatics, and hydrogen bonding.
von Freiherr Sass et al. (2023)