› 5nt2

X-ray diffraction
4.26Å resolution

Function and Biology Details

Reactions catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
ATP + [ISG15] + [protein]-lysine = AMP + diphosphate + [protein]-N-ISGyllysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137080 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Non-structural protein 1 Chains: C, D, E, F
Molecule details ›
Chains: C, D, E, F
Length: 233 amino acids
Theoretical weight: 25.87 KDa
Source organism: Influenza A virus (A/Puerto Rico/8/1934(H1N1))
Expression system: Escherichia coli
UniProt:
  • Canonical: P03496 (Residues: 1-230; Coverage: 100%)
Gene name: NS
Sequence domains: Influenza non-structural protein (NS1)
E3 ubiquitin/ISG15 ligase TRIM25 Chains: A, I, N, V
Molecule details ›
Chains: A, I, N, V
Length: 193 amino acids
Theoretical weight: 22.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14258 (Residues: 190-379; Coverage: 30%)
Gene names: EFP, RNF147, TRIM25, ZNF147

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P1
Unit cell:
a: 73.257Å b: 76.296Å c: 92.072Å
α: 100.04° β: 93.71° γ: 111.14°
R-values:
R R work R free
0.272 0.27 0.309
Expression system: Escherichia coli

Quick links

Citations

31 review citations

RIG-I-like receptors: their regulation and roles in RNA sensing.
Rehwinkel et al. (2020)
30 more

8 mentions without citation

The Central Role of Non-Structural Protein 1 (NS1) in Influenza Biology and Infection.
Rosário-Ferreira et al. (2020)
7 more