› 5ntu

X-ray diffraction
2.58Å resolution

Crystal Structure of human Pro-myostatin Precursor at 2.6 A Resolution

Released:
DOI: 10.2210/pdb5ntu/pdb
PDB entry 5ntu coloured by chain, front view.
PDB entry 5ntu coloured by chain, side view.
PDB entry 5ntu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the human myostatin precursor and determinants of growth factor latency.
Cotton TR, Fischer G, Wang X, McCoy JC, Czepnik M, Thompson TB, Hyvönen M
EMBO J 37 367-383 (2018)
PMID: 29330193

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127148 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Growth/differentiation factor 8 Chains: A, B
Molecule details ›
Chains: A, B
Length: 335 amino acids
Theoretical weight: 37.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14793 (Residues: 43-375; Coverage: 95%)
Gene names: GDF8, MSTN
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand EDO 21 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 168.157Å b: 36.301Å c: 120.448Å
α: 90° β: 104.39° γ: 90°
R-values:
R R work R free
0.217 0.215 0.26
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Structural biology of betaglycan and endoglin, membrane-bound co-receptors of the TGF-beta family.
Kim et al. (2019)
7 more

8 mentions without citation

Structural perspective of BMP ligands and signaling.
Gipson et al. (2020)
7 more