› 5nup

X-ray diffraction
2.9Å resolution

Structural basis for maintenance of bacterial outer membrane lipid asymmetry

Released:
DOI: 10.2210/pdb5nup/pdb
PDB entry 5nup coloured by chain, front view.
PDB entry 5nup coloured by chain, side view.
PDB entry 5nup coloured by chain, top view.
Source organism: Klebsiella pneumoniae
Primary publication:
Structural basis for maintenance of bacterial outer membrane lipid asymmetry.
Abellón-Ruiz J, Kaptan SS, Baslé A, Claudi B, Bumann D, Kleinekathöfer U, van den Berg B
Nat Microbiol 2 1616-1623 (2017)
PMID: 29038444

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102788 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Outer membrane porin Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 344 amino acids
Theoretical weight: 38.03 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: F2VN85 (Residues: 22-365; Coverage: 100%)
Gene names: B6I68_03255, GJJ18_12905, NCTC13635_01536, QIG75_13175, SAMEA3515122_01807, SAMEA3673026_00862, SAMEA4364603_01311, SAMEA4873632_02128, VKR_01565, ompC, ompK36
Sequence domains: Gram-negative porin
Structure domains: Porin
Phospholipid-binding lipoprotein MlaA Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 236 amino acids
Theoretical weight: 26.36 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0W8AQT6 (Residues: 18-253; Coverage: 93%)
Gene names: B6I68_03735, BANRA_01481, BL124_00010915, DW281_04185, EAO17_05790, FXN67_06360, G4V31_07835, GJJ08_007755, GJJ13_007140, GJJ18_12455, GNF00_22240, KPZU09_72170, NCTC11679_01560, NCTC13443_05414, NCTC13465_02422, NCTC204_04024, NCTC5047_06653, NCTC5052_03096, NCTC5053_06125, NCTC8849_04376, NCTC9140_05972, NCTC9601_01045, NCTC9617_05977, NCTC9637_02226, NCTC9645_04093, NCTC9661_02390, QIG75_13635, SAMEA3499874_02731, SAMEA3499901_01895, SAMEA3515122_01898, SAMEA3538658_00758, SAMEA3538828_02283, SAMEA3673026_00766, SAMEA3720909_00232, SAMEA4364603_01402, SAMEA4370542_04060, SAMEA4873597_02024, SAMEA4873632_02225, VKR_01474, mlaA
Sequence domains: MlaA lipoprotein

Ligands and Environments

2 bound ligands:
Ligand C8E 19 x C8E
Ligand LMT 3 x LMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 92.377Å b: 145.24Å c: 232.535Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.241 0.29
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Computational Modeling of Realistic Cell Membranes.
Marrink et al. (2019)
16 more

6 mentions without citation

Host adaptation and convergent evolution increases antibiotic resistance without loss of virulence in a major human pathogen.
Fajardo-Lubián et al. (2019)
5 more