› 5nuq

X-ray diffraction
3.2Å resolution

Structural basis for maintenance of bacterial outer membrane lipid asymmetry

Released:
DOI: 10.2210/pdb5nuq/pdb
PDB entry 5nuq coloured by chain, front view.
PDB entry 5nuq coloured by chain, side view.
PDB entry 5nuq coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for maintenance of bacterial outer membrane lipid asymmetry.
Abellón-Ruiz J, Kaptan SS, Baslé A, Claudi B, Bumann D, Kleinekathöfer U, van den Berg B
Nat Microbiol 2 1616-1623 (2017)
PMID: 29038444

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136404 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Outer membrane porin F Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 340 amino acids
Theoretical weight: 37.11 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P02931 (Residues: 23-362; Coverage: 100%)
Gene names: JW0912, b0929, cmlB, coa, cry, ompF, tolF
Sequence domains: Gram-negative porin
Structure domains: Porin
Probable phospholipid-binding lipoprotein mlaA Chains: G, H
Molecule details ›
Chains: G, H
Length: 235 amino acids
Theoretical weight: 26.3 KDa
Source organism: Serratia marcescens
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand C8E 9 x C8E
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P212121
Unit cell:
a: 115.491Å b: 163.039Å c: 230.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.238 0.306
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Computational Modeling of Realistic Cell Membranes.
Marrink et al. (2019)
14 more

2 mentions without citation

Assembly and Maintenance of Lipids at the Bacterial Outer Membrane.
Lundstedt et al. (2021)
1 more