› 5nvu

Electron Microscopy
15Å resolution

Full length human cytoplasmic dynein-1 in the phi-particle conformation

Released:
DOI: 10.2210/pdb5nvu/pdb
PDB entry 5nvu coloured by chain, front view.
PDB entry 5nvu coloured by chain, side view.
PDB entry 5nvu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM Reveals How Human Cytoplasmic Dynein Is Auto-inhibited and Activated.
Zhang K, Foster HE, Rondelet A, Lacey SE, Bahi-Buisson N, Bird AW, Carter AP
Cell 169 1303-1314.e18 (2017)
PMID: 28602352
Related structures: EMD-3705

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero octadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166039 (preferred)
Entry contents:
14 distinct polypeptide molecules
Macromolecules (14 distinct):
Dynein motor domain Chains: A, B
Molecule details ›
Chains: A, B
Length: 3169 amino acids
Theoretical weight: 269.72 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Dynein tail heavy chain Chain: C
Molecule details ›
Chain: C
Length: 932 amino acids
Theoretical weight: 79.34 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Dynein intermediate chain Chains: D, E
Molecule details ›
Chains: D, E
Length: 350 amino acids
Theoretical weight: 29.8 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Dynein tail heavy chain Chain: F
Molecule details ›
Chain: F
Length: 893 amino acids
Theoretical weight: 76.02 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Dynein light intermediate chain Chain: G
Molecule details ›
Chain: G
Length: 298 amino acids
Theoretical weight: 25.38 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Dynein light intermediate chain Chain: H
Molecule details ›
Chain: H
Length: 295 amino acids
Theoretical weight: 25.12 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
N-terminal dimerization domain Chain: I
Molecule details ›
Chain: I
Length: 125 amino acids
Theoretical weight: 10.66 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
N-terminal dimerization domain Chain: J
Molecule details ›
Chain: J
Length: 124 amino acids
Theoretical weight: 10.57 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
LC8 Chains: K, L
Molecule details ›
Chains: K, L
Length: 85 amino acids
Theoretical weight: 7.25 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Tctex Chain: M
Molecule details ›
Chain: M
Length: 103 amino acids
Theoretical weight: 8.78 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Tctex Chain: N
Molecule details ›
Chain: N
Length: 104 amino acids
Theoretical weight: 8.87 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Intermediate chain N-terminus peptides Chain: O
Molecule details ›
Chain: O
Length: 27 amino acids
Theoretical weight: 2.32 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Intermediate chain N-terminus peptides Chain: P
Molecule details ›
Chain: P
Length: 29 amino acids
Theoretical weight: 2.49 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Robl Chains: Q, R
Molecule details ›
Chains: Q, R
Length: 120 amino acids
Theoretical weight: 10.23 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 15Å
Relevant EMDB volumes: EMD-3705
Expression system: Spodoptera frugiperda

Quick links

Citations

18 review citations

Axonal transport: Driving synaptic function.
Guedes-Dias et al. (2019)
17 more

9 mentions without citation

The cytoplasmic dynein transport machinery and its many cargoes.
Reck-Peterson et al. (2018)
8 more